Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CCDC170 / C6orf97 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109624-100
Catalog Number: LS-G109624
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C6orf97(CCDC170) (NM_025059), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA282P11.1 | C6orf97 | CCDC170
- Origin Species
- Human
- NCBI GenBank
- NM_025059
- SwissProt
- Q8IYT3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 82.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSLDCTSHIA LGAASPAPEE TYDHLSEVPV TREQLNHYRN VAQNARSELA ATLVKFECAQ61SELQDLRSKM LSKEVSCQEL KAEMESYKEN NARKSSLLTS LRDRVQELEE ESAALSTSKI121RTEITAHAAI KENQELKKKV VELNEKLQKC SKENEENKKQ VSKNCRKHEE FLTQLRDCLD181PDERNDKASD EDLILKLRDL RKENEFVKGQ IVILEETINV HEMEAKASRE TIMRLASEVN241REQKKAASCT EEKEKLNQDL LSAVEAKEAL EREVKIFQER LLAGQQVWDA SKQEVSLLKK301SSSELEKSLK ASQDAVTTSQ SQYFSFREKI TALLRGRLSM TGSTEDTILE KIREMDSREE361SRDRMVSQLE AQISELVEQL GKESGFHQKA LQRAQKAENM LETLQGQLTH LEAELVSGGV421LRDNLNFEKQ KYLKFLDQLS QKMKLDQMAA ELGFDMRLDV VLARTEQLVR LESNAVIENK481TIAHNLQRKL KTQKERLESK ELHMSLLRQK IAQLEEEKQA RTALVVERDN AHLTIRNLQK541KVERLQKELN TCRDLHTELK AKLADTNELK IKTLEQTKAI EDLNKSRDQL EKMKEKAEKK601LMSVKSELDT TEHEAKENKE RARNMIEVVT SEMKTLKKSL EEAEKREKQL ADFREVVSQM661LGLNVTSLAL PDYEIIKCLE RLIHSHQHHF VTCACLKDVT TGQERHPQGH LQLLHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
715 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.