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Human C14orf104 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human C14orf104 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all DNAAF2 antibodies →
$220.00each
Catalog Number: LS-G82086-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human C14orf104(DNAAF2) (NM_018139) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14orf104 | CILD10 | DNAAF2 | Kintoun | KTU | PF13 | Protein kintoun
Origin Species
Human
NCBI GenBank
NM_018139
SwissProt
Q9NVR5

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
91.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAKAAASSSL EDLDLSGEEV QRLTSAFQDP EFRRMFSQYA EELTDPENRR RYEAEITALE
61RERGVEVRFV HPEPGHVLRT SLDGARRCFV NVCSNALVGA PSSRPGSGGD RGAAPGSHWS
121LPYSLAPGRE YAGRSSSRYM VYDVVFHPDA LALARRHEGF RQMLDATALE AVEKQFGVKL
181DRRNAKTLKA KYKGTPEAAV LRTPLPGVIP ARPDGEPKGP LPDFPYPYQY PAAPGPRAPS
241PPEAALQPAP TEPRYSVVQR HHVDLQDYRC SRDSAPSPVP HELVITIELP LLRSAEQAAL
301EVTRKLLCLD SRKPDYRLRL SLPYPVDDGR GKAQFNKARR QLVVTLPVVL PAARREPAVA
361VAAAAPEESA DRSGTDGQAC ASAREGEAGP ARSRAEDGGH DTCVAGAAGS GVTTLGDPEV
421APPPAAAGEE RVPKPGEQDL SRHAGSPPGS VEEPSPGGEN SPGGGGSPCL SSRSLAWGSS
481AGRESARGDS SVETREESEG TGGQRSACAM GGPGTKSGEP LCPPLLCNQD KETLTLLIQV
541PRIQPQSLQG DLNPLWYKLR FSAQDLVYSF FLQFAPENKL STTEPVISIS SNNAVIELAK
601SPESHGHWRE WYYGVNNDSL EERLFVNEEN VNEFLEEVLS SPFKQSMSLT PPLIEVLQVT
661DNKIQINAKL QECSNSDQLQ GKEERVNEES HLTEKEYIEH CNTPTTDSDS SIAVKALQID
721SFGLVTCFQQ ESLDVSQMIL GKSQQPESKM QSEFIKEKSA TCSNEEKDNL NESVITEEKE
781TDGDHLSSLL NKTTVHNIPG FDSIKETNMQ DGSVQVIKDH VTNCAFSFQN SLLYDLDTRP
LEQKLISEEDLAANDILDYKDDDDKV

840 aa + 26 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.