LSBio
Human NRDE2 / C14orf102 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NRDE2 / C14orf102 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all NRDE2 antibodies →
$220.00each
Catalog Number: LS-G84925-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NRDE2 (NM_017970) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14orf102 | NRDE2
Origin Species
Human
NCBI GenBank
NM_017970
SwissProt
Q9H7Z3

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
132.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MALFPAFAGL SEAPDGGSSR KELDWLSNPS FCVGSITSLS QQTEAAPAHV SEGLPLTRSH
61LKSESSDESD TNKKLKQTSR KKKKEKKKKR KHQHHKKTKR KHGPSSSSRS ETDTDSEKDK
121PSRGVGGSKK ESEEPNQGNN AAADTGHRFV WLEDIQAVTG ETFRTDKKPD PANWEYKSLY
181RGDIARYKRK GDSCLGINPK KQCISWEGTS TEKKHSRKQV ERYFTKKSVG LMNIDGVAIS
241SKTEPPSSEP ISFIPVKDLE DAAPVTTWLN PLGIYDQSTT HWLQGQGPPE QESKQPDAQP
301DSESAALKAK VEEFNRRVRE NPRDTQLWMA FVAFQDEVMK SPGLYAIEEG EQEKRKRSLK
361LILEKKLAIL ERAIESNQSS VDLKLAKLKL CTEFWEPSTL VKEWQKLIFL HPNNTALWQK
421YLLFCQSQFS TFSISKIHSL YGKCLSTLSA VKDGSILSHP ALPGTEEAMF ALFLQQCHFL
481RQAGHSEKAI SLFQAMVDFT FFKPDSVKDL PTKGQVEFFE PFWDSGEPRA GEKGARGWKA
541WMHQQERGGW VVINPDEDDD EPEEDDQEIK DKTLPRWQIW LAAERSRDQR HWRPWRPDKT
601KKQTEEDCED PERQVLFDDI GQSLIRLSSH DLQFQLVEAF LQFLGVPSGF TPPASCLYLA
661MDENSIFDNG LYDEKPLTFF NPLFSGASCV GRMDRLGYPR WTRGQNREGE EFIRNVFHLV
721MPLFSGKEKS QLCFSWLQYE IAKVIWCLHT KNKKRLKSQG KNCKKLAKNL LKEPENCNNF
781CLWKQYAHLE WLLGNTEDAR KVFDTALGMA GSRELKDSDL CELSLLYAEL EVELSPEVRR
841AATARAVHIL TKLTESSPYG PYTGQVLAVH ILKARKAYEH ALQDCLGDSC VSNPAPTDSC
901SRLISLAKCF MLFQYLTIGI DAAVQIYEQV FAKLNSSVFP EGSGEGDSAS SQSWTSVLEA
961ITLMHTSLLR FHMKVSVYPL APLREALSQA LKLYPGNQVL WRSYVQIQNK SHSASKTRRF
1021FDTITRSAKP LEPWLFAIEA EKLRKRLVET VQRLDGREIH ATIPETGLMH RIQALFENAM
1081RSDSGSQCPL LWRMYLNFLV SLGNKERSKG VFYKALQNCP WAKVLYLDAV EYFPDEMQEI
1141LDLMTEKELR VRLPLEELEL LLED
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1164 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.