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SAMD15 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SAMD15 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SAMD15 antibodies →
$725.00each
Catalog Number: LS-G121698-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C14orf174(SAMD15) (NM_001010860), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SAMD15 | C14orf174 | FAM15A
Origin Species
Human
SwissProt
Q9P1V8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEVPEDYDS GPDEDGELEP ERPELPGLHK LYENAEPDTM AKADSKLPAE IYQEPQPETE
61EEDFKEGEPD SAKNVQLKPG GTSQEGIAKE SKRDVPSETE PGIHQEVKSE TSREMGEFFK
121DLEAPMDETH KESDLEPPEE AKPNVTEDVF LESAMETDPD PVPPTETMSE VSGATVRERN
181LELLEEETEP GVPEESLRVQ HEETGLEPPE QTKQDFPSEK LGESLEETDL QPPKMTKPET
241PEETQRESTE KKRTEPPEQA RLEFLEKEPR KSSEEAGLEP PEETQPEVPE EMQRKATEEK
301GTELPERTKP DFPDHKPRKS TDENVPEPLE EIKLEFPEEE SRKTNEETIL EQSEMMKPES
361PEEIRKSNEK KNPQPPEETG PVLPQEINPQ VEEKTQTKPT EKILELPDET KPRETHVEFS
421KEDRPEPIKS KYSVGNDELE HREPKRGKLS LSDKFRKEYY ALGSLRESEE SIGTHYEFLQ
481PLQKLLNVSE ECSYSDPSES QTELSEFVHE KEVVDLSQEL KERVSEDDET QPEKGTELQF
541EHLNWDPEEV AEWISQLGFP QYKECFITNF ISGRKLIHVN CSNLPQMGIT NFEDMKAISR
601HTQELLEIEE PLFKRSISLP YRDIIGLYLE QKGHTGIKSD SLTLSEFVKA AGLQDYAPEI
661TAPEENEELP CTEP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

674 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.