Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NRDE2 / C14orf102 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G112078-100
Catalog Number: LS-G112078
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NRDE2 (NM_017970), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C14orf102 | NRDE2
- Origin Species
- Human
- NCBI GenBank
- NM_017970
- SwissProt
- Q9H7Z3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 132.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MALFPAFAGL SEAPDGGSSR KELDWLSNPS FCVGSITSLS QQTEAAPAHV SEGLPLTRSH61LKSESSDESD TNKKLKQTSR KKKKEKKKKR KHQHHKKTKR KHGPSSSSRS ETDTDSEKDK121PSRGVGGSKK ESEEPNQGNN AAADTGHRFV WLEDIQAVTG ETFRTDKKPD PANWEYKSLY181RGDIARYKRK GDSCLGINPK KQCISWEGTS TEKKHSRKQV ERYFTKKSVG LMNIDGVAIS241SKTEPPSSEP ISFIPVKDLE DAAPVTTWLN PLGIYDQSTT HWLQGQGPPE QESKQPDAQP301DSESAALKAK VEEFNRRVRE NPRDTQLWMA FVAFQDEVMK SPGLYAIEEG EQEKRKRSLK361LILEKKLAIL ERAIESNQSS VDLKLAKLKL CTEFWEPSTL VKEWQKLIFL HPNNTALWQK421YLLFCQSQFS TFSISKIHSL YGKCLSTLSA VKDGSILSHP ALPGTEEAMF ALFLQQCHFL481RQAGHSEKAI SLFQAMVDFT FFKPDSVKDL PTKGQVEFFE PFWDSGEPRA GEKGARGWKA541WMHQQERGGW VVINPDEDDD EPEEDDQEIK DKTLPRWQIW LAAERSRDQR HWRPWRPDKT601KKQTEEDCED PERQVLFDDI GQSLIRLSSH DLQFQLVEAF LQFLGVPSGF TPPASCLYLA661MDENSIFDNG LYDEKPLTFF NPLFSGASCV GRMDRLGYPR WTRGQNREGE EFIRNVFHLV721MPLFSGKEKS QLCFSWLQYE IAKVIWCLHT KNKKRLKSQG KNCKKLAKNL LKEPENCNNF781CLWKQYAHLE WLLGNTEDAR KVFDTALGMA GSRELKDSDL CELSLLYAEL EVELSPEVRR841AATARAVHIL TKLTESSPYG PYTGQVLAVH ILKARKAYEH ALQDCLGDSC VSNPAPTDSC901SRLISLAKCF MLFQYLTIGI DAAVQIYEQV FAKLNSSVFP EGSGEGDSAS SQSWTSVLEA961ITLMHTSLLR FHMKVSVYPL APLREALSQA LKLYPGNQVL WRSYVQIQNK SHSASKTRRF1021FDTITRSAKP LEPWLFAIEA EKLRKRLVET VQRLDGREIH ATIPETGLMH RIQALFENAM1081RSDSGSQCPL LWRMYLNFLV SLGNKERSKG VFYKALQNCP WAKVLYLDAV EYFPDEMQEI1141LDLMTEKELR VRLPLEELEL LLEDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1164 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.