LSBio
Human Apg7 / ATG7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human Apg7 / ATG7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ATG7 antibodies →
$153.00each
Catalog Number: LS-G83205-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ATG7 (NM_001144912) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Apg7 | APG7L | APG7-LIKE | Autophagy related 7 | ATG7 | Autophagy-related protein 7 | HAGP7 | GSA7
Origin Species
Human
NCBI GenBank
NM_006395
SwissProt
O95352

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MAAATGDPGL SKLQFAPFSS ALDVGFWHEL TQKKLNEYRL DEAPKDIKGY YYNGDSAGLP
61ARLTLEFSAF DMSAPTPARC CPAIGTLYNT NTLESFKTAD KKLLLEQAAN EIWESIKSGT
121ALENPVLLNK FLLLTFAIEA LECAYDNLCQ TEGVTALPYF LIKYDENMVL VSLLKHYSDF
181FQGQRTKITI GVYDPCNLAQ YPGWPLRNFL VLAAHRWSSS FQSVEVVCFR DRTMQGARDV
241AHSIIFEVKL PEMAFSPDCP KAVGWEKNQK GGMGPRMVNL SECMDPKRLA ESSVDLNLKL
301MCWRLVPTLD LDKVVSVKCL LLGAGTLGCN VARTLMGWGV RHITFVDNAK ISYSNPVRQP
361LYEFEDCLGG GKPKALAAAD RLQKIFPGVN ARGFNMSIPM PGHPVNFSSV TLEQARRDVE
421QLEQLIESHD VVFLLMDTRE SRWLPAVIAA SKRKLVINAA LGFDTFVVMR HGLKKPKQQG
481AGDLCPNHPV ASADLLGSSL FANIPGYKLG CYFCNDVVAP GDSTRDRTLD QQCTVSRPGL
541AVIAGALAVE LMVSVLQHPE GGYAIASSSD DRMNEPPTSL GLVPHQIRGF LSRFDNVLPV
601SLAFDKCTAC SSKIWDMSDD ETI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

623 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.