Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Apg7 / ATG7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G126402-100
Catalog Number: LS-G126402
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ATG7 (NM_001144912), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Apg7 | APG7L | APG7-LIKE | Autophagy related 7 | ATG7 | Autophagy-related protein 7 | HAGP7 | GSA7
- Origin Species
- Human
- NCBI GenBank
- NM_006395
- SwissProt
- O95352
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAATGDPGL SKLQFAPFSS ALDVGFWHEL TQKKLNEYRL DEAPKDIKGY YYNGDSAGLP61ARLTLEFSAF DMSAPTPARC CPAIGTLYNT NTLESFKTAD KKLLLEQAAN EIWESIKSGT121ALENPVLLNK FLLLTFAIEA LECAYDNLCQ TEGVTALPYF LIKYDENMVL VSLLKHYSDF181FQGQRTKITI GVYDPCNLAQ YPGWPLRNFL VLAAHRWSSS FQSVEVVCFR DRTMQGARDV241AHSIIFEVKL PEMAFSPDCP KAVGWEKNQK GGMGPRMVNL SECMDPKRLA ESSVDLNLKL301MCWRLVPTLD LDKVVSVKCL LLGAGTLGCN VARTLMGWGV RHITFVDNAK ISYSNPVRQP361LYEFEDCLGG GKPKALAAAD RLQKIFPGVN ARGFNMSIPM PGHPVNFSSV TLEQARRDVE421QLEQLIESHD VVFLLMDTRE SRWLPAVIAA SKRKLVINAA LGFDTFVVMR HGLKKPKQQG481AGDLCPNHPV ASADLLGSSL FANIPGYKLG CYFCNDVVAP GDSTRDRTLD QQCTVSRPGL541AVIAGALAVE LMVSVLQHPE GGYAIASSSD DRMNEPPTSL GLVPHQIRGF LSRFDNVLPV601SLAFDKCTAC SSKIWDMSDD ETITRTRPLEQKLISEEDLAANDILDYKDDDDKV
623 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.