Fluorescent image of U251 cells stained with ATG7 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG7 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG7 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000197548).
Apg7 / ATG7 Antibody (aa494-523)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C156613-400
Catalog Number: LS-C156613
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- Description
- ATG7 antibody LS-C156613 is an unconjugated rabbit polyclonal antibody to ATG7 (Apg7) (aa494-523) from human. It is reactive with human and mouse. Validated for IF, IHC and WB.
- Application
- IF, IHC, IHC-P, WB
- Reactivity
- Hu, Ms
- Predicted Reactivity
- Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Apg7 | APG7L | APG7-LIKE | Autophagy related 7 | ATG7 | Autophagy-related protein 7 | HAGP7 | GSA7
- Recommended Dilution: IF/ICC
- 1:200
- Recommended Dilution: IHC-P
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- O95352
- NCBI GenBank
- NM_006395 | NP_006386.1
- SwissProt
- O95352
Target
- Target
- Human Apg7 / ATG7
- Antigen Species
- Human
- Epitope
- This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 494-523 amino acids from human ATG7.
- Gene Name
- ATG7
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS