Immunofluorescence of U251 cells, using ATG7 Antibody. U251 cells(right) were treated with Chloroquine (50 mu M,16h). Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
Apg7 / ATG7 Antibody (aa540-569)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C156615-400
Catalog Number: LS-C156615
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- Description
- ATG7 antibody LS-C156615 is an unconjugated rabbit polyclonal antibody to human ATG7 (Apg7) (aa540-569). Validated for IF, IHC and WB.
- Application
- IF, IHC, IHC-P, WB
- Reactivity
- Hu
- Predicted Reactivity
- Chicken, Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Apg7 | APG7L | APG7-LIKE | Autophagy related 7 | ATG7 | Autophagy-related protein 7 | HAGP7 | GSA7
- Recommended Dilution: IF/ICC
- 1:200
- Recommended Dilution: IHC-P
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- O95352
- NCBI GenBank
- NM_006395 | NP_006386.1
- SwissProt
- O95352
Target
- Target
- Human Apg7 / ATG7
- Antigen Species
- Human
- Epitope
- This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-569 amino acids from the C-terminal region of human ATG7.
- Gene Name
- ATG7
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS