Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ACCN1 / ASIC2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104022-100
Catalog Number: LS-G104022
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ACCN1(ASIC2) (NM_183377), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ACCN1 | ASIC2a | ASIC2 | Brain sodium channel 1 | Degenerin | ACCN | Acid-sensing ion channel 2 | Mammalian degenerin homolog | MDEG | MDEG1 | MDEG2 | HBNaC1 | BNaC1 | MAMMALIAN DEGENERIN | ASIC2b
- Origin Species
- Human
- NCBI GenBank
- NM_183377
- SwissProt
- Q16515
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSRIGGAGLP AAALTGPGRF RMAREEPAPA ALAAAGQPGG GRGGERALQG PGVARRGRPS61LSRAKLHGLR HMCAGRTAAG GSFQRRALWV LAFCTSFGLL LSWSSNRLLY WLSFPSHTRV121HREWSRQLPF PAVTVCNNNP LRFPRLSKGD LYYAGHWLGL LLPNRTARPL VSELLRGDEP181RRQWFRKLAD FRLFLPPRHF EGISAAFMDR LGHQLEDMLL SCKYRGELCG PHNFSSVFTK241YGKCYMFNSG EDGKPLLTTV KGGTGNGLEI MLDIQQDEYL PIWGETEETT FEAGVKVQIH301SQSEPPFIQE LGFGVAPGFQ TFVATQEQRL TYLPPPWGEC RSSEMGLDFF PVYSITACRI361DCETRYIVEN CNCRMVHMPG DAPFCTPEQH KECAEPALGL LAEKDSNYCL CRTPCNLTRY421NKELSMVKIP SKTSAKYLEK KFNKSEKYIS ENILVLDIFF EALNYETIEQ KKAYEVAALL481GDIGGQMGLF IGASILTILE LFDYIYELIK EKLLDLLGKE EDEGSHDENV STCDTMPNHS541ETISHTVNVP LQTTLGTLEE IACTRTRPLEQKLISEEDLAANDILDYKDDDDKV
563 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.