Western Blot: SIRT1 Antibody (1F3) - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), HeLa (3), HEK293 (4) and A549 (5) cell lysates.
SIRT1 / Sirtuin 1 Antibody (clone 1F3)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse, Non-Human Primate, Rat |
| Format: | Liquid |
$394.00each
Catalog Number: LS-C359279-0.1
Catalog Number: LS-C359279
For bulk orders or custom formulations:
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- Description
- Sirtuin 1 antibody LS-C359279 is an unconjugated mouse monoclonal antibody to Sirtuin 1 (SIRT1) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, ICC, IF, IHC and WB.
- Application
- ELISA, FC, ICC, IF, IHC, IHC-P, WB
- Reactivity
- Hu, Ms, NHP, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- SIR2-like protein 1 | SIR2alpha | SIR2L1 | Sirtuin 1 | Sir2-like 1 | SIRT1 | Sirtuin type 1 | HSIR2 | HSIRT1
- Recommended Dilution: ELISA
- 1:10000
- Recommended Dilution: IF/ICC
- 1:10 - 1:1000|1:10 - 1:1000
- Recommended Dilution: IHC-P
- 1:200 - 1:1000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q96EB6
- NCBI GenBank
- NM_012238 | NP_036370.2
- SwissProt
- Q96EB6
Target
- Target
- Human SIRT1 / Sirtuin 1
- Antigen Species
- Human
- Epitope
- Human SIRT1 / Sirtuin 1. Human SIRT1 / Sirtuin 1
- Gene Name
- SIRT1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 1F3
- Isotype
- IgG1
Immunogen
- Immunogen
- Purified recombinant fragment of human SIRT1 (amino acids: 265-452) expressed in E. coli. [UniProt# Q96EB6]
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.03% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 0.1 Milliliter
- Preservative
- Yes
- Purification Method
- Ammonium Sulfate Precipitation
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS