LSBio
IHC-plus™ Polyclonal Goat anti-Human SIRT1 / Sirtuin 1 Antibody (aa577-590, IHC, WB)

Anti-SIRT1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.

IHC-plus™ Polyclonal Goat anti-Human SIRT1 / Sirtuin 1 Antibody (aa577-590, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Non-Human Primate
Format:Liquid
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$383.00each
Catalog Number: LS-B8356-50

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Overview

Description
Sirtuin 1 antibody LS-B8356 is an unconjugated goat polyclonal antibody to Sirtuin 1 (SIRT1) (aa577-590) from human. It is reactive with human and monkey. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu, NHP
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
SIR2-like protein 1 | SIR2alpha | SIR2L1 | Sirtuin 1 | Sir2-like 1 | SIRT1 | Sirtuin type 1 | HSIR2 | HSIRT1
Recommended Dilution: IHC-P
3.75 µg/ml
Recommended Dilution: WB
0.05 - 0.2 µg/ml
UniProt Number
Q96EB6
SwissProt
Q96EB6

Target

Target
SIRT1 / Sirtuin 1
Antigen Species
Human
Specificity
Human SIRT1. This antibody is expected to recognize both reported isoforms (NP_036370.2; NP_001135970.1).
Epitope
Human SIRT1. This antibody is expected to recognize both reported isoforms (NP_036370.2; NP_001135970.1).
Gene Name
SIRT1

Antibody

Host Species
Goat
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Peptide with sequence C-EKPQEVQTSRNVES, from the internal region of the protein sequence according to NP_036370.2NP_001135970.1.
Immunogen Type
Peptide

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Concentration
0.5 mg/mL
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Ammonium Sulfate Precipitation

Storage & Handling

Storage Conditions
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
TBS
Dysregulated miR-34a-SIRT1-acetyl p65 axis is a potential mediator of immune activation in the colon during chronic simian immunodeficiency virus infection of rhesus macaques. Mohan M, Kumar V, Lackner AA, Alvarez X. Journal of immunology (Baltimore, Md. : 1950). 2015;194:291-306. PubMed: 25452565 PMC: PMC4272908

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