Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Affinity purified anti-Smad3 pS423 pS425 antibody was used at 2.5 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong nuclear staining in the majority of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Polyclonal Rabbit anti-Human SMAD3 Antibody (phospho-Ser423/425, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Western Blot |
| Reactivity: | Bovine, Chicken, Human, Mouse, Pig, Rat, Xenopus, Zebrafish |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745301-25
Catalog Number: LS-C745301
For bulk orders or custom formulations:
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- Description
- SMAD3 antibody LS-C745301 is an unconjugated rabbit polyclonal antibody to SMAD3 (pSer423/425) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IHC and WB.
- Application
- ELISA, FC, IHC, WB
- Reactivity
- Bov, Ck, Hu, Ms, Por, Rt, Xen, Zf
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- HSPC193 | HMAD-3 | HsT17436 | LDS1C | LDS3 | Mad homolog JV15-2 | MAD3 | MADH3 | Mothers against DPP homolog 3 | JV15-2 | SMA- and MAD-related protein 3 | SMAD family member 3 | MAD homolog 3 | SMAD3 | HSMAD3 | Mad protein homolog | SMAD 3
- Recommended Dilution: ELISA
- 1:75000 - 1:300000
- Recommended Dilution: IHC
- 1:500
- Recommended Dilution: WB
- 1:2000 - 1:20000
- UniProt Number
- P84022
- NCBI GenBank
- NM_005902 | NP_005893.1
- SwissProt
- P84022
Target
- Target
- Human SMAD3
- Antigen Species
- Human
- Epitope
- Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
- Gene Name
- SMAD3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-SMAD3 pS423pS425 antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human SMAD3 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.16 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
