NMuMG mouse mammary epithelial cells were probed for the activation of Smad3 by detecting phosphorylation of threonine 179. The cells were either untreated or treated with TGF-beta, transferred to membranes and probed with Anti-SMAD3 pT179 (RABBIT) Antibody. The antibody detects only Smad3 in stimulated cells suggesting detection of phosphorylated SMAD3 at T179.

SMAD3 Antibody (phospho-Thr179)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

$579.00each

Catalog Number: LS-C153854-100

Unit Size

Quantity

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Overview

Description: SMAD3 antibody LS-C153854 is an unconjugated rabbit polyclonal antibody to SMAD3 (pThr179) from human. It is reactive with human and mouse. Validated for ELISA and WB.
Application: ELISA, WB
Reactivity: Hu, Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: HSPC193 | HMAD-3 | HsT17436 | LDS1C | LDS3 | Mad homolog JV15-2 | MAD3 | MADH3 | Mothers against DPP homolog 3 | JV15-2 | SMA- and MAD-related protein 3 | SMAD family member 3 | MAD homolog 3 | SMAD3 | HSMAD3 | Mad protein homolog | SMAD 3
Recommended Dilution: ELISA: 1:15000 - 1:75000
Recommended Dilution: WB: 1:1000
UniProt Number: P84022
NCBI GenBank: NM_005902 | NP_005893.1
SwissProt: P84022

Target

Target: Human SMAD3
Antigen Species: Human
Epitope: Reactivity occurs against human Smad3 pT179 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad2. Reactivity against other phosphorylated Smad family members is not known. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, mouse, rat, pig, dog, and marmoset based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Gene Name: SMAD3

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Anti-SMAD3 pT179 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl