Anti-SMAD3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ SMAD3 Antibody (phospho-Ser423/425)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Bovine, Chicken, Human, Mouse, Pig, Rat, Xenopus, Zebrafish
Format:	Liquid

$526.00each

Catalog Number: LS-B64-50

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Quantity

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Overview

Description: SMAD3 antibody LS-B64 is an unconjugated rabbit polyclonal antibody to SMAD3 (pSer423/425) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Application: ELISA, FC, IHC, IHC-P, WB
Reactivity: Bov, Ck, Hu, Ms, Por, Rt, Xen, Zf
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: HSPC193 | HMAD-3 | HsT17436 | LDS1C | LDS3 | Mad homolog JV15-2 | MAD3 | MADH3 | Mothers against DPP homolog 3 | JV15-2 | SMA- and MAD-related protein 3 | SMAD family member 3 | MAD homolog 3 | SMAD3 | HSMAD3 | Mad protein homolog | SMAD 3
Recommended Dilution: ELISA: 1:15000 - 1:30000
Recommended Dilution: IHC-P: 2.5 µg/ml
Recommended Dilution: WB: 1:2000 - 1:20000
UniProt Number: P84022
NCBI GenBank: NM_005902 | NP_005893.1
SwissProt: P84022

Target

Target: SMAD3
Antigen Species: Human
Specificity: Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Epitope: Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Gene Name: SMAD3

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Anti-SMAD3 pS423pS425 antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human SMAD3 protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1.16 mg/mL
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Recommended Storage Buffer: 0.02 M Potassium Phosphate, 0.15 M NaCl

Characterization of articular cartilage homeostasis and the mechanism of superior cartilage regeneration of MRL/MpJ mice. Zhenhan Deng, Xueqin Gao, Xuying Sun, Sarah Amra, Aiping Lu, Yan Cui, Holger K Eltzschig, Guanghua Lei, Johnny Huard. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2019 Aug;33:8809-8821.

PubMed: 31042406

YAP and TAZ are required for the postnatal development and the maintenance of the structural integrity of the oviduct. Philippe Godin , Mayra Tsoi , Marilène Paquet , Derek Boerboom. Reproduction (Cambridge, England). 2020 Aug;;160:307-318.

PubMed: 32520726

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