LSBio
Polyclonal Rabbit anti-Human SMAD3 Antibody (aa179, Internal, WB)

Western blot of Rabbit Anti-SMAD3 antibody. Lane 1: AML12 unstimulated. Lane 2: AML12 stimulated with TGFB. Lane 3: MEFwt unstimulated. Lane 4: MEFwt stimulated with TGFB. Lane 5: MEF Smad3 KO unstimulated. Lane 6: MEF Smad3 KO stimulated with TGFB. Lane 7: HEK293 Smad3T179A mutant unstimulated. Lane 8: HEK293 Smad3T179A mutant stimulated with TGFB. Lane 9: HEK293 Smad3T179V mutant unstimulated. Lane 10: HEK293 Smad3T179V mutant stimulated with TGFB. Load: 35 ug per lane. Primary antibody: SMAD 3 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 48.1kDa. Other band(s): Smad2pT220.

Polyclonal Rabbit anti-Human SMAD3 Antibody (aa179, Internal, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human
Format:Liquid
See all Human SMAD3 antibodies →
$579.00each
Catalog Number: LS-C153867-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
SMAD3 antibody LS-C153867 is an unconjugated rabbit polyclonal antibody to human SMAD3 (aa179, Internal). Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Hu
Predicted Reactivity
Common Marmoset, Dog, Mouse, Pig, Rat
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
HSPC193 | HMAD-3 | HsT17436 | LDS1C | LDS3 | Mad homolog JV15-2 | MAD3 | MADH3 | Mothers against DPP homolog 3 | JV15-2 | SMA- and MAD-related protein 3 | SMAD family member 3 | MAD homolog 3 | SMAD3 | HSMAD3 | Mad protein homolog | SMAD 3
Recommended Dilution: ELISA
1:15000 - 1:25000
Recommended Dilution: WB
1:1000
UniProt Number
P84022
NCBI GenBank
NM_005902 | NP_005893.1
SwissProt
P84022

Target

Target
Human SMAD3
Antigen Species
Human
Epitope
A BLAST analysis was used to suggest cross reactivity with Smad3 from human, mouse, rat, pig, dog, and marmoset based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Gene Name
SMAD3

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
SMAD3 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.