LSBio
Polyclonal Rabbit anti-Human SMAD3 Antibody (C-Terminus, IHC, WB)

Anti-Smad3 Antibody - Western Blot. Western blot of affinity purified anti-Smad3 antibody shows detection of endogenous Smad3 in both unstimulated and stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF-beta lanes for 1 hour. This reagent recognizes both non-phosphorylated and phosphorylated Smad3 protein. Personal Communication. Ying Zhang, NIH, CCR, Bethesda, MD.

Polyclonal Rabbit anti-Human SMAD3 Antibody (C-Terminus, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Bovine, Chicken, Human, Mouse, Pig, Rat, Xenopus, Zebrafish
Format:Liquid
See all Human SMAD3 antibodies →
$579.00each
Catalog Number: LS-C18974-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
SMAD3 antibody LS-C18974 is an unconjugated rabbit polyclonal antibody to SMAD3 (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Bov, Ck, Hu, Ms, Por, Rt, Xen, Zf
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
HSPC193 | HMAD-3 | HsT17436 | LDS1C | LDS3 | Mad homolog JV15-2 | MAD3 | MADH3 | Mothers against DPP homolog 3 | JV15-2 | SMA- and MAD-related protein 3 | SMAD family member 3 | MAD homolog 3 | SMAD3 | HSMAD3 | Mad protein homolog | SMAD 3
Recommended Dilution: ELISA
1:10000 - 1:40000
Recommended Dilution: IHC-P
1:500 - 1:3000
Recommended Dilution: WB
1:500 - 1:3000
UniProt Number
P84022
SwissProt
P84022

Target

Target
Human SMAD3
Antigen Species
Human
Gene Name
SMAD3

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of human SMAD3 protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1.2 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.