Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Anti-Oct4 Antibody - Western Blot. Western blot of affinity purified anti-Oct4 antibody shows detection of endogenous Oct4 in mouse embryonic fibroblast (MEF) cell lysate (lane 1) and HeLa nuclear extract (HN) (lane 2). The band at ~39 kD (arrow head) corresponds to Oct4. After transfer, the membrane was blocked with 5% BSA. Primary antibody was used at a 1:1000 dilution in PBS containing 5% BSA. The specificity of the antibody was confirmed by peptide competition which blocks reaction of the antibody with Oct4 (lanes 3 and 4, respectively).
Polyclonal Rabbit anti-Human POU5F1 / OCT4 Antibody (IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Horse, Human, Mouse, Non-Human Primate |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C60139-100
Catalog Number: LS-C60139
For bulk orders or custom formulations:
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- Description
- OCT4 antibody LS-C60139 is an unconjugated rabbit polyclonal antibody to OCT4 (POU5F1) from human. It is reactive with human, mouse, horse and other species. Validated for ELISA, IF, IP and WB.
- Application
- ELISA, IF, IHC, IP, WB
- Reactivity
- Hr, Hu, Ms, NHP
- Predicted Reactivity
- Bovine, Cynomolgus Monkey, Dog, Mammal (Other), Non-Human Primate, Pig, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Oct-4 | OCT3 | OCT4 | OTF4 | POU class 5 homeobox 1 | POU5F1 | Octamer-binding protein 3 | Octamer-binding protein 4 | OTF-3 | Oct-3 | OTF3 | OCT3/4
- Recommended Dilution: ELISA
- 1:350000
- Recommended Dilution: WB
- 1:1000 - 1:1500
- UniProt Number
- Q01860
- NCBI GenBank
- NM_002701 | NP_002692.2
- SwissProt
- Q01860
Target
- Target
- Human POU5F1 / OCT4
- Antigen Species
- Human
- Epitope
- This antibody reacts with human and mouse Oct-4 protein. Cross-reactivity with Oct-4 from other species has not been tested. A BLAST analysis was used to suggest additional cross-reactivity with Oct-4 from chimpanzee, macaque and horse based on a 100% homology with the immunizing sequence; and mink, dog, bovine and pig based on a 93% homology.
- Gene Name
- POU5F1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Oct-4 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -20°C.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
