LSBio
Polyclonal Rabbit anti-Human POU5F1 / OCT4 Antibody (aa114-163, IHC, WB)

Anti-POU5F1 / OCT4 antibody IHC of human melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Polyclonal Rabbit anti-Human POU5F1 / OCT4 Antibody (aa114-163, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$383.00each
Catalog Number: LS-B1194-100

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Overview

Description
OCT4 antibody LS-B1194 is an unconjugated rabbit polyclonal antibody to human OCT4 (POU5F1) (aa114-163). Validated for IHC and WB. Cited in 1 publication.
Application
IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Oct-4 | OCT3 | OCT4 | OTF4 | POU class 5 homeobox 1 | POU5F1 | Octamer-binding protein 3 | Octamer-binding protein 4 | OTF-3 | Oct-3 | OTF3 | OCT3/4
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1.25 µg/ml
UniProt Number
Q01860
SwissProt
Q01860

Target

Target
POU5F1 / OCT4
Antigen Species
Human
Specificity
Human POU5F1 / OCT4
Epitope
Human POU5F1 / OCT4
Gene Name
POU5F1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide located between aa114-163 of human POU5F1 (Q01860, NP_002692). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Goat, Rabbit (100%); Marmoset (92%); Dog (91%); Bovine, Pig, Guinea pig (85%); Horse (84%); Cat (83%).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% sodium azide, 2% sucrose
Concentration
1 mg/mL
Volume
100 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A/G Affinity

Storage & Handling

Storage Conditions
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS
Step-by-step protocols for non-viral derivation of transgene-free induced pluripotent stem cells from somatic fibroblasts of multiple mammalian species. Sho Yoshimatsu, Atsushi Yamazaki, Kazuya Edamura, Yuko Koushige, Hisashi Shibuya, Emi Qian, Tsukika Sato, Junko Okahara, Noriyuki Kishi, Toshiaki Noce, Yoshifumi Yamaguchi, Hideyuki Okano. Development, growth & differentiation. 2022 August;64:325-341. PubMed: 35841539

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