LSBio
IHC-plus™ Polyclonal Rabbit anti-Human POU5F1 / OCT4 Antibody (IHC, IF, WB)

Anti-POU5F1 / OCT4 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ Polyclonal Rabbit anti-Human POU5F1 / OCT4 Antibody (IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
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$383.00each
Catalog Number: LS-B4194-50

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Overview

Description
OCT4 antibody LS-B4194 is an unconjugated rabbit polyclonal antibody to human OCT4 (POU5F1). Validated for Flow, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
FC, ICC, IF, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Oct-4 | OCT3 | OCT4 | OTF4 | POU class 5 homeobox 1 | POU5F1 | Octamer-binding protein 3 | Octamer-binding protein 4 | OTF-3 | Oct-3 | OTF3 | OCT3/4
Recommended Dilution: IF/ICC
1:100 - 1:200
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1:500 - 1:3000
UniProt Number
Q01860
SwissProt
Q01860

Target

Target
POU5F1 / OCT4
Antigen Species
Human
Specificity
Human OCT3/4. Predicted cross-reactivity based on amino acid sequence homology: rabbit (85%), bovine (85%), pig (88%), monkey (97%).
Epitope
Human OCT3/4. Predicted cross-reactivity based on amino acid sequence homology: rabbit (85%), bovine (85%), pig (88%), monkey (97%).
Gene Name
POU5F1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.
Immunogen Type
Proprietary / Undisclosed

Format

Conjugate/Label
Unconjugated
Formulation
1X PBS, pH 7, 0.01% Thimerosal, 20% Glycerol, 1% BSA
Concentration
1 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
1X PBS

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