Anti-POU5F1 / OCT4 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHC-plus™ POU5F1 / OCT4 Antibody (aa100-200)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human, Mouse, Pig
Format:	Liquid

$383.00each

Catalog Number: LS-B85-0.1

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Quantity

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Overview

Description: OCT4 antibody LS-B85 is an unconjugated rabbit polyclonal antibody to OCT4 (POU5F1) (aa100-200) from human. It is reactive with human, mouse and porcine. Validated for Flow, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application: FC, ICC, IF, IHC, IHC-F, IHC-P, WB
Reactivity: Hu, Ms, Por
Predicted Reactivity: Bovine
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Oct-4 | OCT3 | OCT4 | OTF4 | POU class 5 homeobox 1 | POU5F1 | Octamer-binding protein 3 | Octamer-binding protein 4 | OTF-3 | Oct-3 | OTF3 | OCT3/4
Recommended Dilution: FC: 1:50 - 1:200
Recommended Dilution: IF/ICC: 1:50 - 1:200
Recommended Dilution: IHC-P: 2.5 µg/ml
Recommended Dilution: WB: 2 µg/ml
UniProt Number: Q01860
NCBI GenBank: NM_002701 | NP_002692.2
SwissProt: Q01860

Target

Target: POU5F1 / OCT4
Antigen Species: Human
Specificity: OCT4 - Embryonic Stem Cell Marker.
Epitope: OCT4 - Embryonic Stem Cell Marker.
Gene Name: POU5F1

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: A synthetic peptide made to an internal portion of the human OCT4 protein sequence (between residues 100-200). Percent identity by BLAST analysis: Bovine, Cat, Porcine (93%).
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: Tris-Glycine, 150 mM NaCl, 0.05% Sodium Azide
Concentration: 1 mg/mL
Volume: 0.1 Milliliter
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: Tris-Glycine, 150 mM NaCl

Stabilization of HIF-1a and HIF-2a, up-regulation of MYCC and accumulation of stabilized p53 constitute hallmarks of CNS-PNET animal model. Malchenko S, Sredni ST, Bi Y, Margaryan NV, Boyineni J, Mohanam I, Tomita T, Davuluri RV, Soares MB. PloS one. 2017;12:e0173106. PubMed: 28249000 PMC: PMC5332108

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