LSBio
Polyclonal Rabbit anti-Human NOTCH1 Antibody (N-Terminus, IHC, WB)

Rabbit anti-Human NOTCH 1 (Cleaved N Terminal) was used at a 1:500 dilution to detect mouse Notch 1 by Western blot. Equivalent amounts of lysates from transiently transfected 293 cells expressing recombinant myc-tagged mouse Notch constructs were electrophoresed and transferred to membrane using standard methods. A reaction with diluted primary antibody was followed by washing; reaction with a 1:10000 dilution of HRP conjugated Gt-a-Rabbit IgG, and color development. Lane M: Mol wt markers. Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc.

Polyclonal Rabbit anti-Human NOTCH1 Antibody (N-Terminus, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$579.00each
Catalog Number: LS-C19109-200

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Overview

Description
NOTCH1 antibody LS-C19109 is an unconjugated rabbit polyclonal antibody to NOTCH1 (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB. Cited in 3 publications.
Application
ELISA, IHC, WB
Reactivity
Hu, Ms, Rt
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Notch 1 | NOTCH1 | TAN1
Recommended Dilution: ELISA
1:20000 - 1:60000
Recommended Dilution: IHC
1:200
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P46531
NCBI GenBank
NM_017617, NP_060087.3
SwissProt
P46531

Target

Target
Human NOTCH1
Antigen Species
Hu
Epitope
This antiserum is directed against human NOTCH 1. Based on the immunogen sequence, we expect this antibody to react as well with mouse and rat NOTCH 1 (100% sequence homology). This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells. Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.
Gene Name
NOTCH1

Antibody

Host Species
Rb
Clonality
Polyclonal

Immunogen

Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
Concentration
90 mg/mL
Volume
200 Microliter
Preservative
Yes
Purification Method
Crude

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl
Aberrant activation of notch signaling in human breast cancer. Stylianou S, Clarke RB, Brennan K. Cancer research. 2006;66:1517-25. PubMed: 16452208
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