Monoclonal Rat anti-Mouse CD90.2 (Mouse) Antibody (clone 30H12, AF647)
Catalog Number: LS-C810655
2 sizes / from $259.00
Antibody: Rat Monoclonal
Applications: Flow Cytometry
Reactivity: Mouse
Format: Liquid
Flow cytometry of mouse thymocytes with Mouse anti-Mouse NOTCH-1:FITC This image was taken for the unconjugated form of this product. Other forms have not been tested.
Monoclonal Mouse anti-Mouse NOTCH1 Antibody (clone mN1A, FITC, IHC)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Frozen |
| Reactivity: | Mouse |
| Format: | Liquid |
$350.00each
Catalog Number: LS-C188303-0.1
Catalog Number: LS-C188303
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- NOTCH1 antibody LS-C188303 is an FITC-conjugated mouse monoclonal antibody to mouse NOTCH1. Validated for Flow and IHC.
- Application
- FC, IHC, IHC-F
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Notch 1 | NOTCH1 | TAN1
- Recommended Dilution: FC
- 0.0423611111111111 - 1:10
- UniProt Number
- P46531
- NCBI GenBank
- NM_017617 | NP_060087.3
- SwissProt
- P46531
Target
- Target
- Mouse NOTCH1
- Antigen Species
- Mouse
- Epitope
- Recognizes Notch 1, one of the four major transmembrane receptors (Notch 1-4) of the Notch signalling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. Notch 1 is expressed in a range of cells including hematopoietic cells in mouse fetal liver, adult thymus and bone marrow. Notch 1 signalling plays a role in follicular differentiation, tissue homeostasis, and in both CD4+ and CD8+ cell maturation in the thymus. Studies have also implicated Notch 1 in the regulation of lymphopoiesis, myelopoiesis, and neurogenesis.
- Gene Name
- NOTCH1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- mN1A
- Isotype
- IgG1
Immunogen
- Immunogen
- Synthetic peptide corresponding to cdc10-NCR region within mouse Notch1
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Fluorescein Isothiocyanate
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide, 1% BSA
- Concentration
- 0.1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
- Recommended Storage Buffer
- PBS
