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Polyclonal Rabbit anti-Human MAP1LC3A / LC3A Antibody (phospho-Ser12, WB)

Immunoblots of phosphorylated LC3 (phospho-LC3) in CHO cell culture. LC3 and LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with anti-phospho-LC3 S12 antibody. LC3 = microtubule-associated protein light chain-3; S12A = replacement of the amino acid position 12 serine of LC3 with alanine. WT = wild type LC3-transfected cell lysates; S12A = LC3 S12A mutant-transfected cell lysates; Empty vector = vector with no LC3 gene. Molecular size: LC3-I = 16kDa, and LC3-II = 14 kDa

Polyclonal Rabbit anti-Human MAP1LC3A / LC3A Antibody (phospho-Ser12, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Dot Blot, Western Blot
Reactivity:Human
Format:Liquid
See all Human MAP1LC3A / LC3A antibodies →
$401.00each
Catalog Number: LS-C158634-400

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Overview

Description
LC3A antibody LS-C158634 is an unconjugated rabbit polyclonal antibody to human LC3A (MAP1LC3A) (pSer12). Validated for DB and WB. Cited in 1 publication.
Application
DB, WB
Reactivity
Hu
Predicted Reactivity
Bovine, Mouse, Rat
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ATG8E | LC3 | LC3A | MAP1BLC3 | MAP1LC3A | MAP1A/1B light chain 3 A | MAP1A/MAP1B LC3 A | MAP1A/MAP1B light chain 3 A | MAP1ALC3
Recommended Dilution: WB
1:1000
UniProt Number
Q9H492
SwissProt
Q9H492

Target

Target
Human MAP1LC3A / LC3A
Antigen Species
Human
Epitope
This LC3C Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S12 of human LC3C.
Gene Name
MAP1LC3A

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen Type
Peptide

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Volume
400 Microliter
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Recommended Storage Buffer
PBS
Rab5 and class III phosphoinositide 3-kinase Vps34 are involved in hepatitis C virus NS4B-induced autophagy. Su WC, Chao TC, Huang YL, Weng SC, Jeng KS, Lai MM. Journal of virology. 2011;85:10561-71. PubMed: 21835792 PMC: PMC3187495

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