Flow Cytometry: LC3 Antibody FACS staining of NTERA-2 cells at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAP1LC3A / LC3A Antibody (N-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$394.00each
Catalog Number: LS-C142708-0.1
Catalog Number: LS-C142708
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- Description
- LC3A antibody LS-C142708 is an unconjugated rabbit polyclonal antibody to LC3A (MAP1LC3A) (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for Flow, IHC and WB.
- Application
- FC, IHC, IHC-P, WB
- Reactivity
- Hu, Ms, Rt
- Predicted Reactivity
- Bovine, Zebrafish
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ATG8E | LC3 | LC3A | MAP1BLC3 | MAP1LC3A | MAP1A/1B light chain 3 A | MAP1A/MAP1B LC3 A | MAP1A/MAP1B light chain 3 A | MAP1ALC3
- Recommended Dilution: FC
- 1:100
- Recommended Dilution: IHC-P
- 1:100
- Recommended Dilution: WB
- 1:2500
- UniProt Number
- Q9H492
- NCBI GenBank
- NM_032514 | NP_115903.1
- SwissProt
- Q9H492
Target
- Target
- Human MAP1LC3A / LC3A
- Antigen Species
- Human
- Epitope
- Although specificity between LC3A and LC3B has not been tested, this was created to a peptide that has 100% identity to LC3A and 62% identity to LC3B.
- Gene Name
- MAP1LC3A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Genomic sequence made to a N-Terminus portion of the human LC3 protein [Swiss-Prot# Q9H492]. Percent identity by BLAST analysis: Rat (100%); Bovine (97%); Zebrafish (94%).
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.05% Sodium Azide, 50% Glycerol, 0.1% BSA
- Concentration
- 0.2 mg/mL
- Volume
- 0.1 Milliliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS