Immunofluorescence of U251 cells, using LC3 Antibody (APG8). U251 cells(right) were treated with Chloroquine (50 mu M,16h). The antibody was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
MAP1LC3A / LC3A Antibody
| Antibody: | Mouse Monoclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- LC3A antibody LS-C165694 is an unconjugated mouse monoclonal antibody to LC3A (MAP1LC3A) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB. Cited in 2 publications.
- Application
- IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- ATG8E | LC3 | LC3A | MAP1BLC3 | MAP1LC3A | MAP1A/1B light chain 3 A | MAP1A/MAP1B LC3 A | MAP1A/MAP1B light chain 3 A | MAP1ALC3
- Recommended Dilution: IF/ICC
- 1:200
- Recommended Dilution: IHC
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q9H492
- NCBI GenBank
- NM_032514 | NP_115903.1
- SwissProt
- Q9H492
Target
- Target
- Human MAP1LC3A / LC3A
- Antigen Species
- Human
- Epitope
- This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).
- Gene Name
- MAP1LC3A
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Concentration
- 0.5 mg/mL
- Volume
- 200 Microliter
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS
Mouse knock-out of IOP1 protein reveals its essential role in mammalian cytosolic iron-sulfur protein biogenesis. Song D, Lee FS. The Journal of biological chemistry. 2011;286:15797-805.
PubMed: 21367862 · PMC: PMC3091189
Mitochondria-lysosome membrane contacts are defective in GDAP1-related Charcot-Marie-Tooth disease. Lara Cantarero , Elena Juárez-Escoto , Azahara Civera-Tregón , María Rodríguez-Sanz, Mónica Roldán, Raúl Benítez , Janet Hoenicka , Francesc Palau. The Breast : official journal of the European Society of Mastology. 2021 Jan;29:3589-3605.
PubMed: 33372681 · PMC: PMC7823109