Fluorescent image of U251 cells stained LC3 (APG8A) antibody.U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated LC3 (APG8A) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
MAP1LC3A / LC3A Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C165695-400
Catalog Number: LS-C165695
For bulk orders or custom formulations:
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- Description
- LC3A antibody LS-C165695 is an unconjugated rabbit polyclonal antibody to human LC3A (MAP1LC3A). Validated for IF and WB.
- Application
- IF, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ATG8E | LC3 | LC3A | MAP1BLC3 | MAP1LC3A | MAP1A/1B light chain 3 A | MAP1A/MAP1B LC3 A | MAP1A/MAP1B light chain 3 A | MAP1ALC3
- Recommended Dilution: IF/ICC
- 1:200
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q9H492
- NCBI GenBank
- NM_032514 | NP_115903.1
- SwissProt
- Q9H492
Target
- Target
- Human MAP1LC3A / LC3A
- Antigen Species
- Human
- Epitope
- This LC3 antibody is generated from rabbits immunized with full-length recombinant human LC3 (APG8a).
- Gene Name
- MAP1LC3A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Ammonium Sulfate Precipitation
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS