LSBio
Polyclonal Goat anti-Human GAPDH Antibody (aa92-103, IHC, IF, WB)

(0.1 ug/ml) staining of Human Tonsil Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Polyclonal Goat anti-Human GAPDH Antibody (aa92-103, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry, Paraffin, Western Blot
Reactivity:Dog, Hamster, Human, Mouse, Non-Human Primate, Pig, Rabbit, Rat
Format:Liquid
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$514.00each
Catalog Number: LS-C20161-100

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Overview

Description
GAPDH antibody LS-C20161 is an unconjugated goat polyclonal antibody to GAPDH (aa92-103) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, Peptide-ELISA and WB. Cited in 3 publications.
Application
ELISA, IF, IHC-P, WB
Reactivity
Can, Ham, Hu, Ms, NHP, Por, Rb, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
A1 40 kd subunit | Activator 1 40 kd subunit | G3PD | GAPD | G3pdh | Rfc40 | Rf-c 40 kd subunit | GAPDH
UniProt Number
P04406
SwissProt
P04406

Target

Target
Human GAPDH
Antigen Species
Human
Epitope
Human GAPDH. GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Gene Name
GAPDH

Antibody

Host Species
Goat
Clonality
Polyclonal

Immunogen

Immunogen
Peptide with sequence C-GVNHEKYDNSLK, from the internal region of the protein sequence according to NP_002037.2NP_001243728.1.
Immunogen Type
Peptide

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Concentration
0.5 mg/mL
Preservative
Yes
Purification Method
Ammonium Sulfate Precipitation

Storage & Handling

Storage Conditions
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
TBS

Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and retinal transplantation. Vugler A, Carr AJ, Lawrence J, Chen LL, Burrell K, Wright A, Lundh P, Semo M, Ahmado A, Gias C, da Cruz L, Moore H, Andrews P, Walsh J, Coffey P. Experimental neurology. 2008;214:347-61.

PubMed: 18926821


Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay. Carr AJ, Vugler A, Lawrence J, Chen LL, Ahmado A, Chen FK, Semo M, Gias C, da Cruz L, Moore HD, Walsh J, Coffey PJ. Molecular vision. 2009;15:283-95.

PubMed: 19204785 · PMC: PMC2635847


The expression of retinal cell markers in human retinal pigment epithelial cells and their augmentation by the synthetic retinoid fenretinide. Carr AJ, Vugler AA, Yu L, Semo M, Coffey P, Moss SE, Greenwood J. Molecular vision. 2011;17:1701-15.

PubMed: 21738400 · PMC: PMC3130725

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