LSBio
IHC-plus™ Polyclonal Goat anti-Human GAPDH Antibody (aa279-290, IHC, IF, WB)

Staining (0.01 ug/ml) of 293 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.

IHC-plus™ Polyclonal Goat anti-Human GAPDH Antibody (aa279-290, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Non-Human Primate
Format:Liquid
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$495.00each
Catalog Number: LS-B9169-50

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Overview

Description
GAPDH antibody LS-B9169 is an unconjugated goat polyclonal antibody to GAPDH (aa279-290) from human. It is reactive with human and monkey. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IF, IHC, IHC-P, WB
Reactivity
Hu, NHP
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
A1 40 kd subunit | Activator 1 40 kd subunit | G3PD | GAPD | G3pdh | Rfc40 | Rf-c 40 kd subunit | GAPDH
Recommended Dilution: IF/ICC
10 µg/ml
Recommended Dilution: IHC-P
1.25 - 2.5 µg/ml
Recommended Dilution: WB
0.01 - 0.5 µg/ml
UniProt Number
P04406
SwissProt
P04406

Target

Target
GAPDH
Antigen Species
Human
Specificity
Human GAPDH. GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Epitope
Human GAPDH. GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Gene Name
GAPDH

Antibody

Host Species
Goat
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Peptide with sequence C-HQVVSSDFNSDT, from the C-Terminus of protein sequence according to NP_002037.2.
Immunogen Type
Peptide

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Concentration
0.5 mg/mL
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Ammonium Sulfate Precipitation

Storage & Handling

Storage Conditions
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
TBS

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