LSBio
IHC-plus™ GAPDH Antibody (clone 1D4)

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

IHC-plus™ GAPDH Antibody (clone 1D4)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Bovine, Human, Mouse, Pig, Rat
Format:Liquid
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$383.00each
Catalog Number: LS-B12855-250

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Overview

Description
GAPDH antibody LS-B12855 is an unconjugated mouse monoclonal antibody to GAPDH from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ICC, IF, IHC, IHC-P, WB
Reactivity
Bov, Hu, Ms, Por, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
A1 40 kd subunit | Activator 1 40 kd subunit | G3PD | GAPD | G3pdh | Rfc40 | Rf-c 40 kd subunit | GAPDH
Recommended Dilution: IF/ICC
1:100
Recommended Dilution: IHC-P
1:50
Recommended Dilution: WB
1:1000
UniProt Number
P04406
SwissProt
P04406

Target

Target
GAPDH
Antigen Species
Human
Epitope
Clone 1D4 recognizes GAPDH specifically both in western blots and in immunocytochemical experiments. On blots clone 1D4 reveals a prominent ~38kDa band, and on cells in tissue culture the antibody stains in a punctate cytoplasmic fashion. clone 1D4 is known to react with GAPDH from human, cow, pig, mouse, rat and other mammals, and also recognizes avian GAPDH. Since GAPDH is one of the most conserved proteins known, it is likely that the antibody is effective on other species also.
Gene Name
GAPDH

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
1D4
Light Chain
Kappa Light Chain
Origin Species
Human

Immunogen

Immunogen
The immunogen used to raise this antibody was extensively purified pig GAPDH.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Tissue Culture Supernatant, 10 mM Sodium Azide
Volume
250 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Hybridoma Supernatant (Crude)

Storage & Handling

Storage Conditions
Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.