LSBio
Polyclonal Goat anti-Bacteria GLUL / Glutamine Synthetase Antibody (WB)

Glutamine Synthetase Polyclonal Antibody-Western blot. Goat anti-Glutamine Synthetase antibody was used to detect Glutamine Synthetase under reducing and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti-goat (1:10K in TBS/MB-070) 1 hr RT. Images were collected using the Bio-Rad VersaDoc System. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Polyclonal Goat anti-Bacteria GLUL / Glutamine Synthetase Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot
Reactivity:Bacteria
Format:Lyophilized
See all Bacteria GLUL / Glutamine Synthetase antibodies →
$1250.00each
Catalog Number: LS-C59227-2

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Overview

Description
Glutamine Synthetase antibody LS-C59227 is an unconjugated goat polyclonal antibody to Glutamine Synthetase (GLUL) from bacteria. It is reactive with bacteria and brevibacterium. Validated for ELISA, IP and WB.
Application
DB, ELISA, IP, WB
Reactivity
Bac
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
GLNS | Glutamate decarboxylase | Glutamate-ammonia ligase | Glutamine synthase | GS | GLUL | Glutamate--ammonia ligase | Glutamine synthetase | PIG43 | PIG59
Recommended Dilution: ELISA
1:5000 - 1:20000
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:500 - 1:5000
UniProt Number
P15104
SwissProt
P15104

Target

Target
Bacteria GLUL / Glutamine Synthetase
Antigen Species
Bacteria
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glutamine Synthetase [Microbial]. Cross reactivity against Glutamine Synthetase from other sources is unknown.
Gene Name
GLUL

Antibody

Host Species
Goat
Clonality
Polyclonal

Immunogen

Immunogen
Glutamine Synthetase [native from brevibacterium species]
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Unconjugated
Format
Lyophilized
Formulation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
90 mg/mL
Volume
2 Milliliter
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Store at 4°C.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.