LSBio
Polyclonal Goat anti-Bacteria GLUL / Glutamine Synthetase Antibody (Lyophilized, HRP, WB)

Goat anti Glutamine Synthetase antibody was used to detect Glutamine Synthetase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat. Images were collected using the BioRad VersaDoc System.

Polyclonal Goat anti-Bacteria GLUL / Glutamine Synthetase Antibody (Lyophilized, HRP, WB)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot
Reactivity:Bacteria
Format:Lyophilized
See all Bacteria GLUL / Glutamine Synthetase antibodies →
$564.00each
Catalog Number: LS-C744740-100

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Overview

Description
Glutamine Synthetase antibody LS-C744740 is an HRP-conjugated goat polyclonal antibody to Glutamine Synthetase (GLUL) from bacteria. It is reactive with bacteria and brevibacterium. Validated for ELISA, IP and WB.
Application
ELISA, IP, WB
Reactivity
Bac
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
GLNS | Glutamate decarboxylase | Glutamate-ammonia ligase | Glutamine synthase | GS | GLUL | Glutamate--ammonia ligase | Glutamine synthetase | PIG43 | PIG59
Recommended Dilution: ELISA
1:2000 - 1:10000
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:500 - 1:2500
UniProt Number
P15104
SwissProt
P15104

Target

Target
Bacteria GLUL / Glutamine Synthetase
Antigen Species
Bacteria
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial]. Cross reactivity against Glutamine Synthetase from other sources is unknown.
Gene Name
GLUL

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Glutamine Synthetase [native from brevibacterium species]
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Horseradish Peroxidase
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Ion Exchange Chromatography

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.