LSBio
Polyclonal Goat anti-Bacteria GLUL / Glutamine Synthetase Antibody (Lyophilized, WB)

Western Blot of Goat anti-Glutamine Synthetase antibody. Lane 1: Glutamine Synthetase Reduced. Lane 2: Glutamine Synthetase Non-Reduced. Load: ~1ug of protein per lane. Primary antibody: Glutamine Synthetase antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 649 conjugated Donkey anti goat. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 53.3 kDa, 56 kDa for Glutamine Synthetase. Other band(s): Glutamine Synthetase splice variants and isoforms.

Polyclonal Goat anti-Bacteria GLUL / Glutamine Synthetase Antibody (Lyophilized, WB)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot
Reactivity:Bacteria
Format:Lyophilized
See all Bacteria GLUL / Glutamine Synthetase antibodies →
$579.00each
Catalog Number: LS-C744698-100

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Overview

Description
Glutamine Synthetase antibody LS-C744698 is an unconjugated goat polyclonal antibody to Glutamine Synthetase (GLUL) from bacteria. It is reactive with bacteria and brevibacterium. Validated for ELISA, IP and WB.
Application
ELISA, IP, WB
Reactivity
Bac
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
GLNS | Glutamate decarboxylase | Glutamate-ammonia ligase | Glutamine synthase | GS | GLUL | Glutamate--ammonia ligase | Glutamine synthetase | PIG43 | PIG59
Recommended Dilution: ELISA
1:5000 - 1:20000
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:500 - 1:5000
UniProt Number
P15104
SwissProt
P15104

Target

Target
Bacteria GLUL / Glutamine Synthetase
Antigen Species
Bacteria
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial]. Cross reactivity against Glutamine Synthetase from other sources is unknown.
Gene Name
GLUL

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Glutamine Synthetase [native from brevibacterium species]
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Unconjugated
Format
Lyophilized
Formulation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Ion Exchange Chromatography

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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