Goat anti Glutamine Synthetase antibody was used to detect Glutamine Synthetase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat. Images were collected using the BioRad VersaDoc System.
GLUL / Glutamine Synthetase Antibody (Liquid, HRP)
Primary AntibodyLSBio Guarantee
| Antibody: | Goat Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot |
| Reactivity: | Bacteria |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C744742-25
Catalog Number: LS-C744742
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- Description
- Glutamine Synthetase antibody LS-C744742 is an HRP-conjugated goat polyclonal antibody to Glutamine Synthetase (GLUL) from bacteria. It is reactive with bacteria and brevibacterium. Validated for ELISA, IP and WB.
- Application
- ELISA, IP, WB
- Reactivity
- Bac
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- GLNS | Glutamate decarboxylase | Glutamate-ammonia ligase | Glutamine synthase | GS | GLUL | Glutamate--ammonia ligase | Glutamine synthetase | PIG43 | PIG59
- Recommended Dilution: ELISA
- 1:2000 - 1:10000
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:500 - 1:2500
- UniProt Number
- P15104
- NCBI GenBank
- NM_002065 | NP_002056.2
- SwissProt
- P15104
Target
- Target
- Bacteria GLUL / Glutamine Synthetase
- Antigen Species
- Bacteria
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial]. Cross reactivity against Glutamine Synthetase from other sources is unknown.
- Gene Name
- GLUL
Antibody
- Host Species
- Goat
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Glutamine Synthetase [native from brevibacterium species]
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Horseradish Peroxidase
- Format
- Liquid
- Formulation
- 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl