Western blot analysis of eNOS (pS1177) expression in A549 (A); HUVEC (B); rat brain (C) whole cell lysates.
NOS3 / eNOS Antibody (phospho-Ser1177)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot |
| Reactivity: | Dog, Human, Mouse, Pig, Rabbit, Rat, Sheep |
| Format: | Liquid |
$269.00each
Catalog Number: LS-C352612-30
Catalog Number: LS-C352612
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- Description
- ENOS antibody LS-C352612 is an unconjugated rabbit polyclonal antibody to eNOS (NOS3) (pSer1177) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IP and WB.
- Application
- ICC, IF, IP, WB
- Reactivity
- Can, Hu, Ms, Por, Rb, Rt, Sh
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Constitutive NOS | CNOS | Endothelial NOS | ECNOS | NOS type III | NOSIII | EC-NOS | ENOS | Nitric oxide synthase 3 | NOS3
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IP
- 1:10 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P29474
- NCBI GenBank
- NM_000603 | NP_000594.2
- SwissProt
- P29474
Target
- Target
- Human NOS3 / eNOS
- Antigen Species
- Human
- Epitope
- Recognizes endogenous levels of eNOS (pS1177) protein.
- Gene Name
- NOS3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human eNOS.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
- Volume
- 30 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Aliquot to avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS