Western blot analysis of lysates from Jurkat cells, using eNOS (Phospho-Thr494) Antibody. The lane on the right is blocked with the phospho peptide.
NOS3 / eNOS Antibody (phospho-Thr494)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$341.00each
Catalog Number: LS-C117346-50
Catalog Number: LS-C117346
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- Description
- ENOS antibody LS-C117346 is an unconjugated rabbit polyclonal antibody to eNOS (NOS3) (pThr494) from human. It is reactive with human, mouse and rat. Validated for Peptide-ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Constitutive NOS | CNOS | Endothelial NOS | ECNOS | NOS type III | NOSIII | EC-NOS | ENOS | Nitric oxide synthase 3 | NOS3
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P29474
- NCBI GenBank
- NM_000603 | NP_000594.2
- SwissProt
- P29474
Target
- Target
- Human NOS3 / eNOS
- Antigen Species
- Human
- Epitope
- eNOS (Phospho-Thr494) Antibody detects endogenous levels of eNOS only when phosphorylated at threonine494.
- Gene Name
- NOS3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Synthetic peptide from human eNOS around the phosphorylation site of Thr494. (AA Range: 462-511)
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150 mM NaCl