LSBio
IHC-plus™ Polyclonal Rabbit anti-Human NOS3 / eNOS Antibody (aa1181-1194, IHC, WB)

Anti-NOS3 / eNOS antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ Polyclonal Rabbit anti-Human NOS3 / eNOS Antibody (aa1181-1194, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Non-Human Primate, Rabbit
Format:Liquid
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$495.00each
Catalog Number: LS-B8720-50

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Overview

Description
ENOS antibody LS-B8720 is an unconjugated rabbit polyclonal antibody to eNOS (NOS3) (aa1181-1194) from human. It is reactive with human, monkey and rabbit. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
IHC, IHC-P, WB
Reactivity
Hu, NHP, Rb
Predicted Reactivity
Bovine, Guinea Pig, Hamster, Mouse, Pig, Rat
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Constitutive NOS | CNOS | Endothelial NOS | ECNOS | NOS type III | NOSIII | EC-NOS | ENOS | Nitric oxide synthase 3 | NOS3
Recommended Dilution: IHC-P
5 µg/ml
UniProt Number
P29474
SwissProt
P29474

Target

Target
NOS3 / eNOS
Antigen Species
Human
Specificity
Recognizes eNOS/NOS III, Mr ~135 kD
Epitope
Recognizes eNOS/NOS III, Mr ~135 kD
Gene Name
NOS3

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 1181-1194 (C-QERQLRGAVPWAFD) of human eNOS/NOS III. The immunizing sequence has 10/11 identical amino acids in mouse, guinea pig, bovine, pig, rat, and dog Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Elephant, Rabbit (100%); Mouse, Rat, Hamster, Panda, Bovine, Pig, Guinea pig (93%); Dog, Bat (86%).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.014 M PB, pH 7.6, 0.175 M NaCl, 0.07% Sodium Azide, 30% Glycerol
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.014 M PB, 0.175 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.