Anti-ENOS antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

IHC-plus™ NOS3 / eNOS Antibody (aa597-611)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunocytochemistry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Bovine, Dog, Guinea Pig, Hamster, Horse, Human, Mammal (Other), Mouse, Non-Human Primate, Rabbit, Rat
Format:	Liquid

$495.00each

Catalog Number: LS-B6341-50

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Overview

Description: ENOS antibody LS-B6341 is an unconjugated rabbit polyclonal antibody to eNOS (NOS3) (aa597-611) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application: ICC, IHC, IHC-P, WB
Reactivity: Bov, Can, GP, Ham, Hr, Hu, Mml, Ms, NHP, Rb, Rt
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Constitutive NOS | CNOS | Endothelial NOS | ECNOS | NOS type III | NOSIII | EC-NOS | ENOS | Nitric oxide synthase 3 | NOS3
Recommended Dilution: IF/ICC: 1:250
Recommended Dilution: IHC-P: 1:200
Recommended Dilution: WB: 1:1000
UniProt Number: P29474
NCBI GenBank: NM_000603 | NP_000594.2
SwissProt: P29474

Target

Target: NOS3 / eNOS
Antigen Species: Human
Specificity: Recognizes human, mouse, rat and dog eNOS (NOS III). Does not cross-react with inducible NOS (iNOS; NOS II) or neuronal NOS (nNOS; NOS I). Detects a band of ~140kD by Western blot. Species cross-reactivity: Human, Mouse, Rat, Dog.
Epitope: Recognizes human, mouse, rat and dog eNOS (NOS III). Does not cross-react with inducible NOS (iNOS; NOS II) or neuronal NOS (nNOS; NOS I). Detects a band of ~140kD by Western blot. Species cross-reactivity: Human, Mouse, Rat, Dog.
Gene Name: NOS3

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: Synthetic peptide corresponding to aa 597-611 (P597YNSSPRPEQHKSYK611C) of bovine endothelial nitric oxide synthase (eNOS; NOS III). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Guinea pig (100%); Pig (87%).
Immunogen Type: Synthetic Peptide (Linear)