LSBio
IHC-plus™ Polyclonal Rabbit anti-Human CENPF / CENP-F Antibody (C-Terminus, IHC, IF, WB)

Anti-CENPF antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500. This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHC-plus™ Polyclonal Rabbit anti-Human CENPF / CENP-F Antibody (C-Terminus, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
See all CENPF / CENP-F antibodies →
$383.00each
Catalog Number: LS-B276-0.1

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Overview

Description
CENP-F antibody LS-B276 is an unconjugated rabbit polyclonal antibody to human CENP-F (CENPF) (C-Terminus). Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Application
IF, IHC, IHC-P, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
AH antigen | CENPF | Centromere protein F | CENF | CENP-F | Kinetochore protein CENPF | Mitosin | CENP-F kinetochore protein | Hcp-1 | PRO1779
Recommended Dilution: IF/ICC
1:750
Recommended Dilution: IHC-P
1:500
Recommended Dilution: IP
1:50
Recommended Dilution: WB
1:1500
UniProt Number
P49454
SwissProt
P49454

Target

Target
CENPF / CENP-F
Antigen Species
Human
Specificity
C-terminus of human CENP-F
Epitope
CENP-F.
Gene Name
CENPF

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
A bacterial fusion protein from the C-Terminus of CENP-F.
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.05% Sodium Azide, 50% Glycerol
Concentration
2.5 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Impaired 53BP1/RIF1 DSB mediated end-protection stimulates CtIP-dependent end resection and switches the repair to PARP1-dependent end joining in G1. Bakr A, Köcher S, Volquardsen J, Petersen C, Borgmann K, Dikomey E, Rothkamm K, Mansour WY. Oncotarget. 2016;7:57679-57693.

PubMed: 27494840 · PMC: PMC5295381


Loss of PTEN-assisted G2/M checkpoint impedes homologous recombination repair and enhances radio-curability and PARP inhibitor treatment response in prostate cancer. Mansour WY, Tennstedt P, Volquardsen J, Oing C, Kluth M, Hube-Magg C, Borgmann K, Simon R, Petersen C, Dikomey E, Rothkamm K. Scientific reports. 2018 March;8:3947.

PubMed: 29500400 · PMC: PMC5834544


Depletion of Akt1 and Akt2 Impairs the Repair of Radiation-Induced DNA Double Strand Breaks via Homologous Recombination. Mohammadian Gol T, Rodemann HP, Dittmann K. International journal of molecular sciences. 2019 December;20:E6316.

PubMed: 31847370 · PMC: PMC6941063

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