Detection of Human CENP-F/Mitosin by Immunocytochemistry. Samples: Acetone-fixed HeLa cells. Antibody: Affinity purified rabbit anti-CENP-F/Mitosin used at a dilution of 1:1000 (1 ug/mg). Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.
CENPF / CENP-F Antibody (aa1800-1850)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunoprecipitation |
| Reactivity: | Human |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C288403-10
Catalog Number: LS-C288403
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- Description
- CENP-F antibody LS-C288403 is an unconjugated rabbit polyclonal antibody to human CENP-F (CENPF) (aa1800-1850). Validated for ICC, IF and IP.
- Application
- ICC, IF, IP
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AH antigen | CENPF | Centromere protein F | CENF | CENP-F | Kinetochore protein CENPF | Mitosin | CENP-F kinetochore protein | Hcp-1 | PRO1779
- Recommended Dilution: IF/ICC
- 1:500 - 1:2000|1:500 - 1:2000
- UniProt Number
- P49454
- NCBI GenBank
- NM_016343 | NP_057427.3
- SwissProt
- P49454
Target
- Target
- Human CENPF / CENP-F
- Antigen Species
- Human
- Epitope
- Region between residue 1800 and 1850 of human centromere protein F (Mitosin) using the numbering given in entry AAA82889.1 (GeneID 1063).
- Gene Name
- CENPF
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 1063 corresponding to amino acids between 1800 and 1850 of UniprotID: P49454
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate