Monoclonal Mouse Biotin Antibody
Catalog Number: LS-C127042-200
200 µg / $755.00
Antibody: Mouse Monoclonal
Applications: Enzyme-Linked Immunosorbent Assay
Format: Liquid
Anti-CENPF antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC-plus™ Monoclonal Mouse anti-Human CENPF / CENP-F Antibody (clone 14C10 1D8, aa1759-2093, IHC, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
$383.00each
Catalog Number: LS-B3046-50
Catalog Number: LS-B3046
For bulk orders or custom formulations:
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- Description
- CENP-F antibody LS-B3046 is an unconjugated mouse monoclonal antibody to human CENP-F (CENPF) (aa1759-2093). Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
- Application
- IF, IHC, IHC-F, IHC-P, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- AH antigen | CENPF | Centromere protein F | CENF | CENP-F | Kinetochore protein CENPF | Mitosin | CENP-F kinetochore protein | Hcp-1 | PRO1779
- Recommended Dilution: IHC-P
- 10 µg/ml
- Recommended Dilution: WB
- 1:500
- UniProt Number
- P49454
- NCBI GenBank
- NM_016343 | NP_057427.3
- SwissProt
- P49454
Target
- Target
- CENPF / CENP-F
- Antigen Species
- Human
- Specificity
- Reacts with human Mitosin.
- Epitope
- Reacts with human Mitosin.
- Gene Name
- CENPF
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 14C10 1D8
- Origin Species
- Human
Immunogen
- Immunogen
- Protein fragment expressed in E. coli corresponding to amino acids 1759-2093.
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Formulation
- PBS
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Animal Free
- No
- Carrier-Free
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Keep as concentrated solution. For short term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
Impaired 53BP1/RIF1 DSB mediated end-protection stimulates CtIP-dependent end resection and switches the repair to PARP1-dependent end joining in G1. Bakr A, Köcher S, Volquardsen J, Petersen C, Borgmann K, Dikomey E, Rothkamm K, Mansour WY. Oncotarget. 2016;7:57679-57693.
PubMed: 27494840 · PMC: PMC5295381
Loss of PTEN-assisted G2/M checkpoint impedes homologous recombination repair and enhances radio-curability and PARP inhibitor treatment response in prostate cancer. Mansour WY, Tennstedt P, Volquardsen J, Oing C, Kluth M, Hube-Magg C, Borgmann K, Simon R, Petersen C, Dikomey E, Rothkamm K. Scientific reports. 2018 March;8:3947.
PubMed: 29500400 · PMC: PMC5834544