Monoclonal Mouse Biotin Antibody
Catalog Number: LS-C127042-200
200 µg / $755.00
Antibody: Mouse Monoclonal
Applications: Enzyme-Linked Immunosorbent Assay
Format: Liquid
Anti-CENPF antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC-plus™ Monoclonal Mouse anti-Human CENPF / CENP-F Antibody (aa1759-2093, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$526.00each
Catalog Number: LS-B3157-50
Catalog Number: LS-B3157
For bulk orders or custom formulations:
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- Description
- CENP-F antibody LS-B3157 is an unconjugated mouse monoclonal antibody to human CENP-F (CENPF) (aa1759-2093). Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
- Application
- IHC, IHC-F, IHC-P, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- AH antigen | CENPF | Centromere protein F | CENF | CENP-F | Kinetochore protein CENPF | Mitosin | CENP-F kinetochore protein | Hcp-1 | PRO1779
- Recommended Dilution: IHC-F
- 10 µg/ml
- Recommended Dilution: IHC-P
- 10 µg/ml
- Recommended Dilution: IP
- 1 - 10 µg/ml
- Recommended Dilution: WB
- 1 - 10 µg/ml
- UniProt Number
- P49454
- NCBI GenBank
- NM_016343 | NP_057427.3
- SwissProt
- P49454
Target
- Target
- CENPF / CENP-F
- Antigen Species
- Human
- Specificity
- Recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.
- Epitope
- Recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.
- Gene Name
- CENPF
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4
- Concentration
- 1 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at -70°C for up to 1 year. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
Identification and mapping of a linear epitope of centromere protein F using monoclonal antibodies. Welner S, Trier NH, Houen G, Hansen PR. Journal of peptide science : an official publication of the European Peptide Society. 2013;19:95-101. PubMed: 23297090