Western blot detection of Hsp27 in Hela, HsHela, A431, COS7, Lncap and MCF7 cell lysates and using Hsp27 mouse mAb (1:1000 diluted). Predicted band size: 23KDa. Observed band size: 27KDa.
HSPB1 / HSP27 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human, Non-Human Primate |
| Format: | Liquid |
$339.00each
Catalog Number: LS-C813004-50
Catalog Number: LS-C813004
For bulk orders or custom formulations:
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- Description
- HSP27 antibody LS-C813004 is an unconjugated mouse monoclonal antibody to HSP27 (HSPB1) from human. It is reactive with human and monkey. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu, NHP
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- 28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | HSPB1 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
- UniProt Number
- P04792
- NCBI GenBank
- NM_001540 | NP_001531.1
- SwissProt
- P04792
Recommended Dilution
| Application | Dilution |
|---|---|
| ELISA | 1:10000 - 1:20000 |
| WB | 1:500 - 1:2000 |
Target
- Target
- Human HSPB1 / HSP27
- Antigen Species
- Human
- Epitope
- This antibody detects endogenous levels of Hsp27 and does not cross-react with related proteins.
- Gene Name
- HSPB1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Isotype
- IgG1
- Origin Species
- Human
Immunogen
- Immunogen
- Purified recombinant human Hsp27 protein fragments expressed in E.coli.
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.02% Sodium Azide, 50% Glycerol, 0.5% BSA
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS