Western blot analysis of lysates from HUVEC cells treated with TNF 20ng/ml 30', using HSP27 (Phospho-Ser82) Antibody. The lane on the right is blocked with the phospho peptide.
HSPB1 / HSP27 Antibody (phospho-Ser82)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$341.00each
Catalog Number: LS-C117379-50
Catalog Number: LS-C117379
For bulk orders or custom formulations:
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- Description
- HSP27 antibody LS-C117379 is an unconjugated rabbit polyclonal antibody to HSP27 (HSPB1) (pSer82) from human. It is reactive with human, mouse and rat. Validated for Peptide-ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | HSPB1 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P04792
- NCBI GenBank
- NM_001540 | NP_001531.1
- SwissProt
- P04792
Target
- Target
- Human HSPB1 / HSP27
- Antigen Species
- Human
- Epitope
- HSP27 (Phospho-Ser82) Antibody detects endogenous levels of HSP27 only when phosphorylated at serine82.
- Gene Name
- HSPB1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Synthetic peptide from human HSP27 around the phosphorylation site of Ser82. (AA Range: 48-97)
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150 mM NaCl