LSBio
PathPlus™ HSPB1 / HSP27 Antibody (clone G3.1)

Anti-HSPB1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

PathPlus™ HSPB1 / HSP27 Antibody (clone G3.1)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$526.00each
Catalog Number: LS-B383-50

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Overview

Description
HSP27 antibody LS-B383 is an unconjugated mouse monoclonal antibody to human HSP27 (HSPB1). Validated for ELISA, IHC and WB. Cited in 3 publications.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | HSPB1 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
Recommended Dilution: ELISA
1:10000 - 1:50000
Recommended Dilution: IHC-P
1.25 µg/ml
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
P04792
NCBI GenBank
NM_001540 | NP_001531.1
SwissProt
P04792

Target

Target
HSPB1 / HSP27
Antigen Species
Human
Specificity
Reacts specifically with HSP27 in human tissues and cell lines. MCF-7 cells are recommended as a positive control. Cross reactivity with hsp27 from other mammalian sources is likely. No cross reactivity occurs with HSP70, HSP90 or HSP104.
Epitope
Reacts specifically with HSP27 in human tissues and cell lines. MCF-7 cells are recommended as a positive control. Cross reactivity with hsp27 from other mammalian sources is likely. No cross reactivity occurs with HSP70, HSP90 or HSP104.
Gene Name
HSPB1

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
G3.1
Light Chain
Kappa Light Chain
Origin Species
Human

Immunogen

Immunogen
This HSP27 monoclonal antibody was produced by repeated immunizations with a prokaryotic recombinant protein corresponding to the full length human hsp27 protein.
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.5 M NaCl

Chromosomal assignments of human 27-kDa heat shock protein gene family. McGuire SE, Fuqua SA, Naylor SL, Helin-Davis DA, McGuire WL. Somatic cell and molecular genetics. 1989;15:167-71.

PubMed: 2538929

A cDNA for the estradiol-regulated 24K protein: control of mRNA levels in MCF-7 cells. Moretti-Rojas I, Fuqua SA, Montgomery RA, McGuire WL. Breast cancer research and treatment. 1988;11:155-63.

PubMed: 3401605

Estradiol stimulates synthesis of a major intracellular protein in a human breast cancer cell line (MCF-7). Edwards DP, Adams DJ, McGuire WL. Breast cancer research and treatment. 1981;1:209-23.

PubMed: 7348573

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