LSBio
IHC-plus™ HSPB1 / HSP27 Antibody (clone 5D12-A12)

Anti-HSPB1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHC-plus™ HSPB1 / HSP27 Antibody (clone 5D12-A12)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
See all HSPB1 / HSP27 antibodies →
$383.00each
Catalog Number: LS-B7450-50

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Overview

Description
HSP27 antibody LS-B7450 is an unconjugated mouse monoclonal antibody to human HSP27 (HSPB1). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IHC, IHC-P, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | HSPB1 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
0.25 - 0.5 µg/ml
UniProt Number
P04792
NCBI GenBank
NM_001540 | NP_001531.1
SwissProt
P04792

Target

Target
HSPB1 / HSP27
Antigen Species
Human
Specificity
Detects a 27kD protein corresponding to the molecular mass of hsp27 on SDS PAGE immunoblots. There is no cross-reactivity to alphaBcrystallin.
Epitope
Detects a 27kD protein corresponding to the molecular mass of hsp27 on SDS PAGE immunoblots. There is no cross-reactivity to alphaBcrystallin.
Gene Name
HSPB1

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
5D12-A12
Light Chain
Kappa Light Chain
Origin Species
Human

Immunogen

Immunogen
Human Hsp27.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.