LSBio
DYKDDDDK Tag Antibody (clone 29E4.G7, HRP)

Western Blot of Mouse antibody for the detection of FLAG® conjugated proteins Monoclonal Antibody Peroxidase Conjugated. Lane 1: FLAG® Positive Control Lysates. Load: 10 µg per lane. Primary antibody: FLAG® Monoclonal Antibody Peroxidase Conjugated at 1:1,000 for overnight at 4°C. Secondary antibody: none.

DYKDDDDK Tag Antibody (clone 29E4.G7, HRP)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Format:Lyophilized
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$475.00each
Catalog Number: LS-C154016-100

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Overview

Description
DYKDDDDK Tag antibody LS-C154016 is an HRP-conjugated mouse monoclonal antibody to DYKDDDDK Tag. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA
1:10000 - 1:50000
Recommended Dilution: IHC
1:500 - 1:2500
Recommended Dilution: WB
1:10000

Target

Target
DYKDDDDK Tag
Epitope
This antibody is directed against the FLAG™ epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Antibody

Host Species
Ms
Clonality
Monoclonal
Clone
29E4.G7
Isotype
IgG2a
Light Chain
κ

Immunogen

Immunogen
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Immunogen Type
SP-Lin

Format

Conjugate/Label
HRP
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Crude

Storage & Handling

Storage Conditions
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.