LSBio
DYKDDDDK Tag Antibody (clone 29E4.G7, HRP)

Western Blot of Mouse antibody for the detection of FLAG® conjugated proteins Monoclonal Antibody Peroxidase Conjugated. Lane 1: FLAG® Positive Control Lysates. Load: 10 µg per lane. Primary antibody: FLAG® Monoclonal Antibody Peroxidase Conjugated at 1:1,000 for overnight at 4°C. Secondary antibody: none.

DYKDDDDK Tag Antibody (clone 29E4.G7, HRP)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Format:Lyophilized
See all DYKDDDDK Tag antibodies →
$475.00each
Catalog Number: LS-C154016-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
DYKDDDDK Tag antibody LS-C154016 is an HRP-conjugated mouse monoclonal antibody to DYKDDDDK Tag. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA
1:10000 - 1:50000
Recommended Dilution: IHC
1:500 - 1:2500
Recommended Dilution: WB
1:10000

Target

Target
DYKDDDDK Tag
Epitope
This antibody is directed against the FLAG™ epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
29E4.G7
Isotype
IgG2a
Light Chain
Kappa Light Chain

Immunogen

Immunogen
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Horseradish Peroxidase
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.