IR700DX - Western Blot. Western Blot showing detection of alpha tubulin from HeLa and NIH/3T3. Protein was run on a 4-20% gel, transferred to 0.45 micron nitrocellulose. After blocking with 1% BSA-TTBS (MB-013, diluted to 1X) 30 min at 20C, primary antibody was used at 1:2500 overnight at 4C. IRDye700DX secondary antibody was used at 1:20000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070) and imaged on the LiCor Odyssey imaging system. Arrow indicates correct 50 kD molecular weight position expected for alpha tubulin. This image was taken for the unconjugated form of this product. Other forms have not been tested.