LSBio
Monoclonal Mouse DYKDDDDK Tag Antibody (clone 29E4.G7, Agarose, WB)

FLAG-Agarose Conjugated - SDS-PAGE of Simple Purification. FLAG- Agarose Conjugated Antibody. Briefly, a 500 ul amount of E. coli cell lysate was added to (bind to) 100 ul Anti-FLAG resin for 1 hour at room temperature. After washing 3 x with 750 ul PBS, bound FLAG-protein was eluted in 200 ul of 0.1 M glycine, pH 2.5 followed with neutralization. Cell lysate, flow through and eluate were loaded on SDS gel for quantification. Lanes (6 ul per lane): M) Molecular Weight markers. 1) Cell lysate before purification. 2) Flow through (used cell lysate). 3) Purified FLAG-tagged recombinant protein (arrowhead).

Monoclonal Mouse DYKDDDDK Tag Antibody (clone 29E4.G7, Agarose, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Immunoprecipitation, Western Blot
Format:Liquid
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$631.00each
Catalog Number: LS-C154020-1

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Overview

Description
DYKDDDDK Tag antibody LS-C154020 is an Agarose-conjugated mouse monoclonal antibody to DYKDDDDK Tag. Validated for IP and WB.
Application
IP, WB
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies

Target

Target
DYKDDDDK Tag
Epitope
Anti-DYKDDDDK Affinity Gel binds the FLAG® epitope tag sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to the amino terminal, carboxy terminal or internal locations of targeted recombinant proteins expressed in transfected or transformed cells. D-Y-K-D-D-D-D-K peptide is recommended for competitive elution to recover fusion protein (see protocol).

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
29E4.G7
Isotype
IgG2a
Light Chain
Kappa Light Chain

Immunogen

Immunogen
Anti-DYKDDDDK Affinity Gel antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG® epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Agarose Bead
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Volume
1 Milliliter
Preservative
Yes

Storage & Handling

Storage Conditions
Store at 4°C.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.