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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AGO2 / EIF2C2 Antibody (clone 2E12‑1C9) IHC‑plus™ LS‑B6224
Note: This antibody replaces LS-C133724
EIF2C2 antibody LS-B6224 is an unconjugated mouse monoclonal antibody to human EIF2C2 (AGO2). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6224-50
50 µg (1 mg/ml)
Unavailable

Popular AGO2 / EIF2C2 Products

Western blot Image
Species: Human, Mouse, Rat
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, Flow Cytometry, ELISA
Antibody
Species: Human, Bovine, Pig, Rabbit
Applications: Western blot
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA, Chromatin Immunoprecipitation

Product Description

EIF2C2 antibody LS-B6224 is an unconjugated mouse monoclonal antibody to human EIF2C2 (AGO2). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About AGO2 / EIF2C2
Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. AF121255 AAF13034.2

AGO2 Antibody, Argonaute2 Antibody, Argonaute 2 Antibody, EIF-2C 2 Antibody, EIF2C2 Antibody, EIF2C 2 Antibody, HAgo2 Antibody, PPD Antibody, Protein slicer Antibody, Q10 Antibody, PAZ Piwi domain protein Antibody, Protein argonaute-2 Antibody


Specifications

Target
Human AGO2 / EIF2C2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [2E12-1C9]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3 - 5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
AGO2 / EIF2C2 antibody was raised against eIF2C2 (AAH07633, 1 a.a. ~ 377 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human EIF2C2
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Western Blot with the recombinant protein used as the immunogen.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-EIF2C2 / AGO2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6224 concentration 3 ug/ml.
Anti-EIF2C2 / AGO2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6224 concentration 3 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to EIF2C2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to EIF2C2 on HeLa cell. [antibody concentration 10 ug/ml]

Immunohistochemistry

Anti-EIF2C2 / AGO2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6224 concentration 3 ug/ml.
Anti-EIF2C2 / AGO2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6224 concentration 3 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to EIF2C2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to EIF2C2 on HeLa cell. [antibody concentration 10 ug/ml]

Immunohistochemistry

Anti-EIF2C2 / AGO2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6224 concentration 3 ug/ml.
Anti-EIF2C2 / AGO2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6224 concentration 3 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to EIF2C2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to EIF2C2 on HeLa cell. [antibody concentration 10 ug/ml]

Immunohistochemistry

Anti-EIF2C2 / AGO2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6224 concentration 3 ug/ml.
Anti-EIF2C2 / AGO2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6224 concentration 3 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to EIF2C2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to EIF2C2 on HeLa cell. [antibody concentration 10 ug/ml]


Requested From: 
Date Requested: 6/25/2018

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